oxiselecttm ages elisa kit  (Cell Biolabs Inc)

Journal: Frontiers in Plant Science

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

doi: 10.3389/fpls.2026.1796014

Figure Lengend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the resistant genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-avr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s45) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Article Snippet: 3-nitrotyrosine (3-NT) levels were quantified using the OxiSelectTM Nitrotyrosine ELISA Kit (Cell Biolabs) according to the manufacturer’s instructions.

Techniques: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

Journal: Frontiers in Plant Science

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

doi: 10.3389/fpls.2026.1796014

Figure Lengend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the susceptible genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-vr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s32) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Article Snippet: 3-nitrotyrosine (3-NT) levels were quantified using the OxiSelectTM Nitrotyrosine ELISA Kit (Cell Biolabs) according to the manufacturer’s instructions.

Techniques: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

Journal: Scientific Reports

Article Title: UV-C LED wavelength effects on inactivation kinetics, DNA damage and membrane integrity in drinking water indicator bacteria

doi: 10.1038/s41598-026-44556-8

Figure Lengend Snippet: Fluorescence microscopy images illustrating membrane integrity and DNA organization in stationary-phase E. coli ( a ) and E. faecium ( b ) before and after UV-C LED exposure at 265 nm (UV fluence of 14 mJ/cm²).From the left side, the first column shows phase contrast microscopy images (grayscale), the second shows membrane staining with FM4-64 (red), the third displays DNA stained with DAPI (cyan), and the fourth shows the merged images of FM4-64 and DAPI. These images show that, under these conditions, most cells retain apparent membrane integrity while displaying UV-induced alterations in DAPI-stained nucleoids. A larger field of view for the same conditions is presented in Figure in supplementary information section.

Article Snippet: The DNA samples were diluted to 4 μg/mL and an enzyme-linked immunosorbent assay (OxiSelectTM UV-Induced DNA Damage ELISA Kit, CPD Quantification, Cell Biolabs, Inc, USA) was used to quantify the presence of CPDs in the DNA samples by comparing its absorbance with the absorbance of a CPD-DNA standard calibration curve.

Techniques: Fluorescence, Microscopy, Membrane, Staining